Biochemist how-to
How to Purify a Protein
Pure proteins give clean data.
Quick answer
Pure proteins give clean data. In short: express; lyse; run, then complete the remaining steps below.
Step by step
Express the protein.
Lyse cells.
Run affinity chromatography.
Run size exclusion.
Check purity on a gel.
Measure concentration.
Safety reminders for biochemists
- Follow biosafety rules
- Handle chemicals safely
- Wear PPE
- Dispose of waste properly
Key terms
Hypothesis · Peer review · Grant · Protocol · Replication · Protein: see the full biochemist glossary.