Biological Technician how-to

How to Use Aseptic Technique

Clean technique prevents contamination.

Updated By the Career Education Institute Curriculum Team
Quick answer

Clean technique prevents contamination. In short: disinfect; arrange; work, then complete the remaining steps below.

Step by step

  1. Disinfect the biosafety cabinet.

  2. Arrange materials.

  3. Work in the center of the cabinet.

  4. Avoid passing over open containers.

  5. Keep lids off for minimal time.

  6. Disinfect after work.

Safety reminders for biological technicians

  • Follow biosafety level rules
  • Wear PPE
  • Dispose of biohazards correctly
  • Never mouth-pipette

PCR · Aseptic technique · Cell culture · Assay · Gel electrophoresis · Centrifuge: see the full biological technician glossary.

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